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Title 

Development of a new type of recombinant hyaluronidase using a hexahistidine; possibilities and challenges in commercialization

Authors 

C ParkY K SongYoung-Hyun KimY JungYoung-Ho ParkBong-Seok SongT EomJ S KimS H KimJi-Su KimSun-Uk KimSang-Rae LeeE Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2019

Citation 

Journal of Microbiology and Biotechnology

Keywords 

Recombinantaffinity chromatographyenzymehyaluronic acidhyaluronidase

Abstract 

Hyaluronidases enhance therapeutic drug transport by breaking down the hyaluronan barrier to lymphatic and capillary vessels, facilitating their tissue absorption. Commercially available hyaluronidases are bovine in origin; however, they pose risks such as bovine spongiform encephalopathy. The present study aimed to develop a novel, highly active hyaluronidase and assess its function. Therefore, in order to find the most efficient active hyaluronidase, we produced several shortened hyaluronidases with partial removal of the N- or C-terminal regions. Moreover, we created an enzyme that connected six histidines onto the end of the hyaluronidase C-terminus. This simplified subsequent purification using Ni2+ affinity chromatography, making it feasible to industrialize this highly active recombinant hyaluronidase which exhibited catalytic activity equal to that of the commercial enzyme. Therefore, this simple and effective isolation method could increase the availability of recombinant hyaluronidase for research and clinical purposes.

URI 

https://doi.org/10.4014/jmb.1905.05049

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-10-29


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