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Title 

A method to isolate DNA sequences that are promoter-active in Escherichia coli and in yeast

Authors 

Ju Won KwakJiyoung KimO J YooMoon Hi Han

Publisher 

Elsevier

Issue Date 

1987

Citation 

Biochemical and Biophysical Research Communications, vol. 149, no. 3, pp. 846-851

Keywords 

bacterial DNAchloramphenicolfungal DNADNA vectordrug resistanceescherichia coliisolation and purificationplasmidpromoter regionsaccharomyces cerevisiae

Abstract 

A method convenient for isolation of DNA sequences capable of directing gene transcription in both organisms of E. coli and yeast is described. The method is composed of sequential steps of phenotypic selection for chloramphenicol resistance, first in E. coli and then in yeast. A series of promoter-probe, shuttle plasmid vectors between yeast and E. coli were constructed and utilized in the method.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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