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Title 

Simple enzyme immunoassay for the simultaneous measurement of whole choriogonadotropin molecules and free beta-subunits in sera of women with abnormal pregnancies or tumors of the reproductive system

Authors 

Myung Ja ChoiIn Seong ChoeHae Kyung KangJong Soon LeeTai Wha Chung

Publisher 

American Association for Clinical Chemistry

Issue Date 

1991

Citation 

Clinical Chemistry, vol. 37, no. 5, pp. 673-677

Keywords 

protein subunitenzyme immunoassayovary tumorplacenta tumorpregnancy disordertrophoblastic tumoruterus tumorchorionic gonadotropin, beta subunit, humanimmunoenzyme techniquespregnancy

Abstract 

A multiple enzyme immunoassay (multi-EIA) was developed to quantify whole-molecule human choriogonadotropin (w-hCG) and free hCG β-subunits (hCG-β) simultaneously. A clone of a specific monoclonal antibody was coupled to solid phase; two other clones of different monoclonal antibodies were conjugated to horseradish peroxidase (HRP; EC 1.11.1.7) and alkaline phosphatase (AP; EC 3.1.3.1), respectively. These two enzyme conjugates were mixed together to measure w-hCG or hCG-β, depending on the selection of the enzyme substrate. To measure w-hCG and hCG-β simultaneously, both enzyme substrates were used with the blended enzyme conjugates. The assay is simple and reproducible, and can be completed within 2 h with high specificity and sensitivity. We measured w-hCG and hCG-β in the sera of women with abnormal pregnancies and in patients with tumors of the reproductive system, and observed different hCG-β/w-hCG ratios in patients with various types of trophoblastic tumors.

ISSN 

0009-9147

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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