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Title 

Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus

Authors 

Hyo Jeong HongAe Kyung KimChun Jeih RyuSung Sup ParkHong Keun ChungKi Sun KwonKil Lyong KimJiyoung KimMoon Hi Han

Publisher 

Elsevier

Issue Date 

1992

Citation 

Gene, vol. 121, no. 0, pp. 331-335

Keywords 

cDNA cloningHBVimmunoglobulinnucleotide sequencepassive immunoprophylaxisrecombinant DNAsurface antigencomplementary dnamonoclonal antibody

Abstract 

Binding specificity of a monoclonal antibody (mAb) (κ, γ 2b) H8 which can react with the pre-S2 peptide of hepatitis B virus (HBV) determined by Western blot analyses. From the hybridoma cell line secreting mAb H8, poly(A)+ RNA was prepared and used as a template for cDNA synthesis and cloning. Full-length cDNAs coding for the heavy and κ light chains of the mAb were cloned from the cDNA library and characterized by nucleotide (nt) sequence analyses and N-terminal amino acid sequencing. The sequence analyses revealed that both heavy and light chain-specific cDNAs are functional, and the variable regions of the heavy and light chains are members of mouse heavy chain subgroup III(c) and light chain group I, respectively. Comparison of the nt sequences with mouse immunoglobulin genes listed in the GenBank data base show that the cDNAs have not been previously reported. The cDNAs will be used for the construction of a therapeutic antibody for HBV infection.

ISSN 

0378-1119

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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