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Title 

Selection of protoclones for high yields of indole alkaloids from suspension cultures of Catharanthus roseus and the qualitative analysis of the compounds by LC-MS

Authors 

K H JungSang Soo KwakSuk Weon KimC Y ChoiG S HeoJang Ryol Liu

Publisher 

Springer

Issue Date 

1992

Citation 

Biotechnology Techniques, vol. 6, no. 4, pp. 305-308

Abstract 

To produce economically important indole alkaloids by cell culture, we have selected protoclones of Catharanthus roseus for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. Protoclone VPC-10 produced catharanthine at 5.9 μg/g fresh wt of cells after 10 days of culture, although the original cell line did not produce it at a level detectable by HPLC. Under the same conditions, protoclone VPC-15 produced ajmalicine at 133.6 μg/g, which was about 3 times the productivity of the original cell line. In addition, the indole alkaloids were qualitatively confirmed by LC-MS.

ISSN 

0951-208X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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