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Title 

Rapid and simple purification of human immunodeficiency virus 1 epitope gp41

Authors 

Mi Jin SohnSeo Hee ChoWon-Hee JangYoung-Hae ChongSang Uk NhamYoung Ik Lee

Publisher 

Elsevier

Issue Date 

1993

Citation 

Journal of Virological Methods, vol. 41, no. 1, pp. 93-100

Keywords 

gp41human immunodeficiency virus-1purificationvirus glycoproteinhuman immunodeficiency virus 1nonhumanprotein purificationepitopesHIV envelope protein gp41HIV-1

Abstract 

In order to develop a reliable and inexpensive serodiagnostic method, part of the transmembrane glycoprotein gene of HIV-1, gp41', (HIV-env 548-646) was cloned into an expression vector, pCT10 with a sequence encoding a hydroxylamine cleavage site and with a part of Lac Z gene (Lac 2'': 834 base pairs) as a fusion partner. Overexpression of Lac Z''-gp41' was induced in E. coli and the gp41' fusion protein was purified to homogeneity by centrifugation, hydroxylamine cleavage and an ion-exchange chromatography. Western blot analysis and enzyme-linked immunosorbant assay (ELISA) using the purified gp41 fragment showed high sensitivity and specificity of gp41 as an antigen to detect anti HIV-1 antibodies in testing human sera. These results suggest that this simple and rapid purification method is reliable for obtaining a large quantity of purified gp41'.

ISSN 

0166-0934

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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