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Title 

High frequency somatic embryogenesis and plant regeneration in tissue cultures of various genotypes of maize(Zea mays L.)

 

다양한 계통의 옥수수에서 체세포배발생을 통한 효율적인 식물체 재분화

Authors 

Won Joong ChungNam Hi SongSung Rsn MinMyung K. KimKwang-W. LeeJang Ryol Liu

Issue Date 

1993

Citation 

Korean Journal of Plant Tissue Culture, vol. 20, no. 4, pp. 221-226

Keywords 

immature earsimmature tasselsplant tissue culturezea mays

Abstract 

Somatic embryogenesis and plant regeneration was obtained from immature inflorescence segments of maize (Zea mays L.). Immature tassels and ears (0.5-3.0 cm) were excised, cut into segments (1 - 2 mm), and place onto media supplemented with varying concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), sucrose and l-proline. After 2-3 weeks, the segments produced compact, nodular, white, embryogenic callus as well as soft, granular, friable, non-embryogenic callus. Embryogenic callus further developed somatic embryos with distinct scutellar- and coleoptile-like structures, which produced normal, fertile plants with no apparent morphological abnormalities. Maximum embryogenic callus formation was observed when segments, cut from 0.6-1.0 cm inflorescences, were cultured on Murashige and Skoog (MS) medium with 2 mg/l 2,4-D, 3% sucrose, and 24 mM l-proline. Although embryogenic callus production was observed in all inbreds tested, significant genotype effects were apparent. The inbreds A619, H99, LH39, LH39, MBS501, MS71, Oh43, U94 and U98 all displayed high frequencies of embryogenic callus production (45-80% of cultured segments) and plant regeneration (up to 50% of callus pieces transferred). Thus, immature inflorescences can be used as explants for high frequency somatic embryogenesis and plant regeneration from several commercially-important maize inbreds.

ISSN 

1015-5880

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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