상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Detection of β-gluosidase activity in polyacrylamide gels with esculin as substrate

Authors 

Ki Sun KwonJ LeeH G KangY C Hah

Publisher 

American Society for Microbiology

Issue Date 

1994

Citation 

Applied and Environmental Microbiology, vol. 60, no. 12, pp. 4584-4586

Keywords 

esculinenzyme substrate complexpolyacrylamide gel electrophoresis

Abstract 

β-Glucosidase can be located after nondenaturing polyacrylamide gel electrophoresis by incubating the gel with 0.1% esculin and 0.03% ferric chloride. The esculetin released from esculin by β-glucosidase action reacts with ferric ion to produce a black band, corresponding to the β-glucosidase, against the transparent background.

ISSN 

0099-2240

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)