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Title 

Overexpression and purification of full-length acidic fibroblast growth factor in E. coli

Authors 

Sang Uk NhamHyo-Soon KimYoung Ik Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

1994

Citation 

Biotechnology Letters, vol. 16, no. 7, pp. 661-666

Keywords 

acidic fibroblast growth factorcomplementary dnacell inclusioncloneescherichia coligene amplificationgene expressionmitogenesispurification

Abstract 

A full-length aFGF cDNA was cloned and inserted into a T7 expression vector for heterologous expression in E. coli. For high level production of aFGF, an optimal condition for overexpression (incubation at 42°C for 2 hours) and a simple purification procedure employing in vitro refolding and an affinity chromatography was established. The production yield was 40-50 μg/mg of inclusion body preparation and purified aFGF showed mitogenic activity.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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