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Title 

Molecular cloning and characterization of the aklavinone 11-hydroxylase gene of Streptomyces peucetius subsp. caesius ATCC 27952

Authors 

Young Soo HongC K HwangSoon Kwang HongY H KimJung Joon Lee

Publisher 

American Society for Microbiology

Issue Date 

1994

Citation 

Journal of Bacteriology, vol. 176, no. 22, pp. 7096-7101

Keywords 

aklavinoneaklavinone 11 hydroxylasegene mutationgene sequencegenetic transcriptionmolecular cloningstreptomycesstreptomyces peucetiusaryl hydrocarbon hydroxylasescloning, molecular

Abstract 

The gene encoding aklavinone 11-hydroxylase of Streptomyces peucetius subsp. caesius ATCC 27952 was cloned and sequenced. The deduced amino acid sequence of the gene contains at least two common motifs of well-conserved amino acid sequences of several flavin-type bacterial hydroxylases. The hydroxylase gene is apparently transcribed from a single transcriptional start point. The phenotype of a dnrF mutant generated by gene disruption supports the idea that the dnrF gene encodes aklavinone 11-hydroxylase.

ISSN 

0021-9193

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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