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Title 

Enzymatic synthesis of levan by Zymomonas mobilis levansucrase overexpressed in Escherichia coli

Authors 

Ki Bang SongSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

1994

Citation 

Biotechnology Letters, vol. 16, no. 12, pp. 1305-1310

Keywords 

levanlevansucraseescherichia colisynthesiszymomonas mobilis

Abstract 

Levansucrase gene from Zymomonas mobilis was expressed efficiently in Escherichia coli and the overproduced recombinant levansucrase amounted to 40% of the total cell protein. Using E. coli lysate, levan was synthesized in a sucrose-based medium enzymatically with the conversion yields of up to 46% from fructose liberated in 25 hrs of incubation. More levan was formed at lower temperatures in the reaction mixture, whereas higher temperatures were favoured for the accumulation of free fructose or short chain oligosaccharides.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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