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Title 

Expression of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae

Authors 

Dong-Hoon LeeJin-Byung ParkJin-Ho SeoEui Sung ChoiSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

1994

Citation 

Biotechnology Letters, vol. 16, no. 7, pp. 667-670

Keywords 

hirudinculture mediumfungus culturegene expressionrecombinant plasmidsaccharomyces cerevisiaesaccharomyces

Abstract 

Fed-batch fermentations were performed to produce hirudin, an anticoagulant protein, from recombinant S. cerevisiae. The structural gene coding for hirudin was combined with the GAL10 promoter for controlled expression and the MFα1 signal sequence for secretion to the growth medium. Control of galactose concentration in a fed-batch mode of operation yielded 110 g/L of final cell density and 260 mg/L of maximum hirudin concentration in the medium, which corresponds to a 3.5-fold increase in cell density and a 4.1-fold enhancement in hirudin concentration compared with the batch fermentation.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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