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Title 

Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus

Authors 

Byung Sik ShinSeung Hwan ParkSoo Keun ChoiBon Tag KooSung-Taik LeeJeong Il Kim

Publisher 

American Society for Microbiology

Issue Date 

1995

Citation 

Applied and Environmental Microbiology, vol. 61, no. 6, pp. 2402-2407

Keywords 

bacillus thuringiensisgene locationbacterial proteinsgene expression

Abstract 

DNA dot blot hybridizations with a cryV-specific probe and a cryI- specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific probe hybridized to 12 of the B. thuringiensis strains examined. Most of the cryV-positive strains also hybridized to the cryI-specific probe, indicating that the cryV genes are closely related to cryI genes. Two cryV-type genes, cryV1 and cryV465 were cloned from B. thuringiensis subsp. kurstaki HD-1 and B. thuringiensis subsp. entomocidus BP465, respectively, and their nucleotide sequences were determined. The CryV1 protein was toxic to Plutella xylostella and Bombyx mori, whereas the CryV465 protein was toxic only to Plutella xylostella.

ISSN 

0099-2240

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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