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Title 

Chemical synthesis and cloning of human β-endorphin gene in Escherichia coli

Authors 

Myung Hee KimHyun Ju LeeK T Min

Publisher 

Humana Press

Issue Date 

1995

Citation 

Applied Biochemistry and Biotechnology, vol. 50, no. 1, pp. 35-43

Keywords 

Β-endorphinescherichia colihuman geneoligonucleotide synthesisrecombinant DNAbeta endorphingeneticshumanmolecular cloning

Abstract 

Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.

ISSN 

0273-2289

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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