상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Sequences responsible for transcription termination of the mouse gastrin gene

Authors 

Moon Jong NohSun Jung KimYong Koo KangOok Joon Yoo

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1995

Citation 

Biochemistry and Molecular Biology International, vol. 35, no. 6, pp. 1205-1213

Keywords 

gastringene sequencegenetic transfectionmouserna transcriptiontranscription terminationbase sequencegastrinsmolecular sequence datapeptide chain termination, translational

Abstract 

To identify the transcription termination elements in the mouse gastrin gene, we examined RNA transcripts after stable transfection of gastrin expression plasmids into the NIH3T3 cell line. The GT-repeat region at the 3'-flanking sequence of the mouse gastrin gene acted as a transcription terminator. When the GT-repeat unit was deleted from its site, the effect of termination disappeared. Further experiment, using serial deletion mutants, revealed that the 56-38 nucleotide upstream region from the GT-repeat unit also participated in transcription termination. We propose that the upstream region of the GT-repeat unit might be recognized as a pause site by the RNA polymerase II, and an abnormal DNA structure, derived from the GT-repeat unit, might function as a blockage of polymerase processivity.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)