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Title 

Overproduction of Serratia marcescens metalloprotease(SMP) from the recombinant Serratia marcescens strains

Authors 

K S KimK S ParkS M ByunJae Gu PanY C Shin

Publisher 

Springer Verlag (Germany)

Issue Date 

1995

Citation 

Biotechnology Letters, vol. 17, no. 5, pp. 497-502

Keywords 

recombinant plasmidserratia marcescens

Abstract 

To overproduce Serratia marcescens metalloprotease (SMP), various recombinant plasmids encoding SMP gene were constructed and the SMP productivities from the recombinant S. marcescens strains were examined. The recombinant S. marcescens strains indispensably required proteinaceous substrates such as casein for the extracellular production of SMP. We obtained maximum 9,100 U/ml of SMP from the culture supernatant of S. marcescens ATCC27117 containing a regulatory plasmid pTSP2 encoding SMP gene fused with a strong trc99a promoter and its repressor gene lacI(q), which is about 23 times higher than that of wild type strain. SMP produced from the recombinant S. marcescens (pTSP2) was 88.3% of total extracellular proteins.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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