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Title 

High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli

Authors 

Soo-Hyung KangKyu-Heum NaJang-Hyeon ParkChoong-Il ParkSe-Yong LeeYoung Ik Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

1995

Citation 

Biotechnology Letters, vol. 17, no. 7, pp. 687-692

Keywords 

recombinant granulocyte colony stimulating factoranimal cellanimal experimentanimal modelbacterium culturecell proliferationcontrolled studyescherichia coligene expressionsubcutaneous drug administration

Abstract 

A human granulocyte colony-stimulating factor (hG-CSF) gene was synthesized and inserted into a trp expression vector for overexpression in E. coli. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabilished. The final productivity of hG-CSF was 500~600 mg per εe culture, and the purified hG-CSF showed the proliferation of neutrophils in vivo assays.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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