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Title 

Production of hirudin by recombinant Saccharomyces cerevisiae in a membrane-recycle fermentor

Authors 

Jin-Byung ParkYoung-Eun KweonSang Ki RheeJin-Ho Seo

Publisher 

Springer Verlag (Germany)

Issue Date 

1995

Citation 

Biotechnology Letters, vol. 17, no. 10, pp. 1031-1036

Keywords 

hirudinrecombinant dna technologyrecombinant genesaccharomyces cerevisiae

Abstract 

Recombinant Saccharomyces cerevisiae was employed to continuously produce hirudin in a membrane cell recycle fermenter. The gene coding for the anticoagulant protein was combined with the GAL10 promoter for controlled expression and the MF α1 signal sequence for secretion to the fermentation broth. A dilution rate of 0.1h-1 yielded a maximum hirudin concentration of 59mg/l with a specific hirudin concentration of 2.4mg/g cell mass among rates studied ranging from 0.05h-1 to 0.3h-1. Cell bleeding gave the same fermentation results as cell recycle fermentation without cell bleeding. The productivity of the cell recycle fermentation process was 6.0mg hirudin/l·hr, corresponding to a 1.7-fold increase compared with a conventional continuous culture.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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