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Title 

B-lymphocyte-stimulating polysaccharide from mushroom Phellinus linteus

Authors 

Kyung-Sik SongSoo Muk ChoJae Hoon LeeHwan Mook KimSang Bae HanKyung-soo KohIck Dong Yoo

Publisher 

Pharmaceutical Society of Japan

Issue Date 

1995

Citation 

Chemical & Pharmaceutical Bulletin, vol. 43, no. 12, pp. 2105-2108

Keywords 

B- lymphocyteimmunostimulatorphellinus linteuspolysaccharide protein complexpolysaccharideantibody producing cellantibody productionb lymphocyte activationmushroomB-Lymphocytes

Abstract 

Hot water extract prepared from the mycelial culture of mushroom Phellinus linteus stimulated polyclonal antibody production in an in vitro culture system. The active fraction PLP was purified from the extract ca. 1030-fold by ethanol precipitation followed by DEAE-cellulose and gel permeation chromatography. PLP contained 13.2% (w/w) peptide and 82.5% (w/w) carbohydrate. About 6.8% (w/w) of the total carbohydrate was uronic acid. The molecular weight distribution of PLP was found to be nearly homogeneous (153 kDa) in gel permeation HPLC analysis. Neutral sugar composition analysis revealed Ara (7.0%), Xyl (3.7%), Glc (21.1%), Gal (24.1%) and Man (44.2%). Uronic acid was identified as a glucuronic acid by gas chromatography. Ten amino acids were detected and Asp and Glu were the major components. In our may system, the half-maximal concentration of PLP for B-lymphocyte stimulation was ca. 3μg/ml. Partial acid hydrolysis as well as sodium periodate treatment of PLP decreased the activity significantly, suggesting that both the full molecular size and the sugar moiety were essential. However, proteinase K treatment for up to 48 h did not affect the activity.

ISSN 

0009-2363

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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