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Title 

Regulation of poly­β­hydroxybutyrate biosynthesis by nicotinamide nucleotide in Alcaligenes eutrophus

Authors 

In Young LeeMi Kyung KimHo Nam ChangYoung Hoon Park

Publisher 

Wiley-Blackwell

Issue Date 

1995

Citation 

FEMS Microbiology Letters, vol. 131, no. 0, pp. 35-39

Keywords 

alcaligenes eutrophusnicotinamide nucleotidepoly-β-hydroxybutyrate biosynthesisregulationpoly(3 hydroxybutyric acid)reduced nicotinamide adenine dinucleotide phosphbiosynthesisgenetic regulationalcaligenes

Abstract 

Poly-β-hydroxybutyrate biosynthesis was studied in Alcaligenes eutrophus under various nutrient-limiting conditions. When the cells were cultivated in nitrogen-limited media, both the levels of NAD(P)H and the ratios of NAD(P)H/NAD(P) were higher than those under nitrogen-sufficient conditions. The specific poly-β-hydroxybutyrate production rate was found to increase with the values of both NADH/NAD and NADPH/NADP, indicating that poly-β-hydroxybutyrate synthesis is directly regulated by the ratios of nicotinamide nucleotides. The effects of nicotinamide nucleotides on poly-β-hydroxybutyrate biosynthesis was investigated with regard to enzyme kinetics. Citrate synthase activity was significantly inhibited by NADH and NADPH, indicating that poly-β-hydroxybutyrate accumulation could be enhanced by facilitating the metabolic flux of acetyl-CoA to poly-β-hydroxybutyrate synthetic pathway. It was also found that cellular NADPH was a limiting substrate for NADPH-linked reductase, controlling the overall biosynthetic activity of poly-β-hydroxybutyrate in this strain.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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