상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Characterization of a DNA fragment carrying the raw starch-digesting α-amylase and salt-dependent α-amylase genes from Bacillus circulans F-2

Authors 

Chul Ho Kim

Publisher 

Wiley-Blackwell

Issue Date 

1995

Citation 

FEMS Microbiology Letters, vol. 126, no. 0, pp. 133-138

Keywords 

α-Amylasebacillus circulansdifferent amylase genedifferential zymogramgenetic isomerraw starch-digesting α-amylase genesalt dependent α-amylase geneamylasedna fragmentpriority journal

Abstract 

A 5.4 kb HindIII DNA fragment carrying the gene encoding raw starch-digesting a-amylase (RSDA), has been previously cloned from Bacillus circulans F-2 and expressed in Escherichia coli [Kim et al. (1990) Biochim. Biophys. Acta 1048, 2233-2238]. Interestingly, when the cell extract of E. coli harboring a plasmid carrying this fragment was incubated with 1 M NaCl, it exhibited about 10 times higher enzyme activity than when assayed without NaCl. Differential zymograms showed two different amylase activities: one for RSDA and the other for a salt-dependent α-amylase (SDA). Even though RSDA activity was detected without NaCl, SDA activity was detected only in high concentrations of NaCl. SDA activity was fully detected at above 1 M NaCl. Results from subcloning of the genes, fractionation analysis of cell extracts, and immunological assays clearly suggested that the two amylases are genetically distinct and that genes for both enzymes are closely linked on the 5.4 kb DNA fragment.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)