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Title 

A Novel Aerobic Respiratory Chain-Linked NADH Oxidase System in Zymomonas mobilis

Authors 

YOUNG JAE KIMKi Bang SongSang Ki Rhee

Publisher 

American Society for Microbiology

Issue Date 

1995

Citation 

Journal of Bacteriology, vol. 177, no. 17, pp. 5176-5178

Keywords 

reduced nicotinamide adenine dinucleotide dehydrogenase (quinone)aerobic metabolismelectron transportmembrane vesiclemichaelis constantnonhumanoxidationpriority journalrespiratory chainzymomonas mobilis

Abstract 

Membrane vesicles prepared from Zymomonas mobilis oxidized NADH exclusively, whereas deamino-NADH was little oxidized. In addition, the respiratory chain-linked NADH oxidase system exhibited only a single apparent K(m) value of approximately 66 μM for NADH. The NADH oxidase was highly sensitive to the respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-N- oxide. However, the NADH:quinone oxidoreductase was not sensitive to 2- heptyl-4-hydroxyquinoline-N-oxide and was highly resistant to another respiratory chain inhibitor, rotenone. Electron transfer from NADH to oxygen generated a proton electrochemical gradient (inside positive) in inside-out membrane vesicles. In contrast, electron transfer from NADH to ubiquinone-1 generated no electrochemical gradient. These findings indicate that Z. mobilis possesses only NADH:quinone oxidoreductase lacking the energy coupling site.

ISSN 

0021-9193

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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