상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

A thermostable mutation located at the hydrophobic core of α₁-antitrypsin suppresses the folding defect of the Z type variant

Authors 

Jeong Ho KimKee Nyung LeeGwan-Su YiMyeong Hee Yu

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

1995

Citation 

Journal of Biological Chemistry, vol. 270, no. 15, pp. 8597-8601

Keywords 

serine proteinase inhibitorconformational transitiongenetic variabilityhydrophobicitymutationpolymerizationpriority journalprotein conformationprotein stabilitythermostability

Abstract 

A thermostable mutation, F51L, at the hydrophobic core of human α1- antitrypsin (α1AT) increased the conformational stability of the molecule by decreasing the unfolding rate significantly without altering the refolding rate. The mutation specifically influenced the transition between the native state and a compact intermediate, which retained ~70% of the far-UV CD signal, but which had most of the fluorescence signal already dequenched. The mutant α1AT protein was more resistant than the wild-type protein to the insertion of the tetradecapeptide mimicking the sequence of the reactive center loop, indicating that the mutation increases the closing of the central β-sheet, the A-sheet, in the native state. The F51L mutation enhanced the folding efficiency of the Z-type (E342K) genetic variation, which causes aggregation of the molecule in the liver. It has been shown previously that the aggregation of the Z protein occurs via loop-sheet polymerization, in which the reactive center loop of one molecule is inserted into the opening of the A-sheet of another molecule. Our results strongly suggest that the hydrophobic core of α1AT regulates the opening- closing of the A-sheet and that certain genetic variations that cause opening of the A-sheet can be corrected by inserting an additional stable mutation into the hydrophobic core.

ISSN 

0021-9258

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)