상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Functional human hepatocytes : isolation from small liver biopsy samples and primary cultivation with liver-specific functions

Authors 

Hwan Mook KimSang Bae HanByung Hwa HyunChang-Joon AHNYoung-Nam CHAKyu Shik JeongGoo Taeg Oh

Publisher 

Japanese Society of Toxicoogical Sciences / The Japanese Society of Toxicology

Issue Date 

1995

Citation 

Journal of Toxicological Sciences, vol. 20, no. 0, pp. 565-578

Keywords 

human livermorphologynon-perfusion hepatocytetoxicologyhuman serum albumincell isolationhumanhuman tissueliver biopsyliver cell

Abstract 

Morphologically and functionally intact human hepatocytes were isolated from small liver biopsy samples weighing about 1-2 g by initial digestion with collagenase followed by repeated digestions with trypsin. The usual yield of hepatocytes was greater than 1x107 cells per g of liver sample and cell viability, as judged by dye exclusion test, was routinely over 90%. The isolated human hepatocytes showed intact morphology under scanning electron microscope. Formation of membrane protrusions upon phalloidin addition demonstrated that the actin in isolated hepatocytes was maintained with its structural integrity. The cultured human hepatocytes retained a variety of liver-specific functions which were similarly exhibited by rat hepatocytes isolated using the same procedure. The cultured human hepatocytes exhibited a specific cytochrome P-450 related enzyme activity, and active amino acid uptake that increased upon addition of hormones like glucagon and dexamethasone. Additionally, the cultured human hepatocytes synthesized DNA actively and, human serum albumin, and was found to be responsive to modulation by growth modulating hormones, cytokines and hepatotoxic agents. Based on the profile of activity described above, the presently established conditions for isolation and culturing of human hepatocytes demonstrate that functional liver cells can be obtained from small biopsied liver samples.

ISSN 

0388-1350

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)