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Title 

Characterization of a murine-human chimeric antibody with specificity for the pre-S2 surface antigen of hepatitis B virus expressed in baculovirus-infected insect cell

Authors 

Byung Rae JinChun Jeih RyuSeok Kwon KangMoon Hi HanHyo Jeong Hong

Publisher 

Elsevier

Issue Date 

1995

Citation 

Virus Research, vol. 38, no. 0, pp. 269-277

Keywords 

baculovirus vectorchimeric antibodyexpressionhepatitis B virustunicamycinvirus vectoramino acid sequenceanimal cellantibody specificity

Abstract 

We have produced and characterized a murine-human chimeric antibody with specificity for the pre-S2 surface antigen of hepatitis B virus (HBV) in baculovirus-infected insect cells. Recombinant baculovirus carrying the cDNA coding for the heavy or light chain of the chimeric antibody was constructed and co-infected into insect cells. The chimeric antibody (BV-S2) expressed in the cells was purified by an affinity chromatography on Protein A-Sepharose 4B column and characterized by N-terminal amino acid sequencing, affinity determination for pre-S2 peptide, endoglycosidase digestion and Clq binding assay, which were then compared with those of the chimeric antibody H69K that has the same amino acid sequence as BV-S2, but produced from transfected murine myeloma cells. The N-linked glycosylation of the BV-S2 antibody was also analyzed by culturing the baculovirus-infected cells in the presence of tunicamycin. The results showed that the BV-S2 was secreted following correct removal of the leader peptides, contained N-linked carbohydrate at the heavy chain, and had the same binding affinity and Clq binding ability as H69K, suggesting that the BV-S2 chimeric antibody is functional and thus may be useful in the prevention of HBV infection.

ISSN 

0168-1702

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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