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Title 

Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences

Authors 

Kwang Lae HoeMi-Sun WonOok-Joon YooHyang Sook Yoo

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1996

Citation 

Biochemistry and Molecular Biology International, vol. 39, no. 1, pp. 127-135

Keywords 

GATA factorlow stringency screeningS. pombeval-tRNAzinc fingertranscription factorvaline transfer rnazinc finger proteinamino acid sequencemolecular cloning

Abstract 

By low stringency screening of a λ-Shizosaccharomyces pombe genomic library, we have cloned a GATA factor homologous gene, gaf2+, within a 3.1-kb EcoRI fragment. The gaf2 ORF predicts a protein of Mr 61 kDa consisting of intronless 564 amino acids corresponding to 1,692 bp. Gaf2 has two zinc-fingers as Urbs1 of Ustilago maydis, whereas most of fungal GATA factors have only one zinc-finger. The separation between two zinc-fingers of Gaf2 is rather long. In addition to gaf2, the sequence analysis revealed a Val-tRNA gene in the 3′-flanking region of gaf2. Northern blot analysis indicated that the gaf2 gene is transcribed constitutively irrespective of the nitrogen source in a medium.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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