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Title 

Cloning and characterization of cDNA for human adenylate kinase 2A

Authors 

Yong Hee LeeJae Wha KimIn Ae LeeHo Bum KangYong Kyung ChoeHee Gu LeeJong-Seok LimHyo Joon KImChankyu ParkIn Seong Choe

Publisher 

International Union of Biochemistry and Molecular Biology

Issue Date 

1996

Citation 

Biochemistry and Molecular Biology International, vol. 39, no. 4, pp. 833-842

Keywords 

AK activitycDNAexpressionhuman adenylate kinase 2Arecombinant proteinadenylate kinasehumanhuman tissuemolecular cloningnonhuman

Abstract 

We have isolated and characterized a cDNA clone encoding human adenylate kinase 2A (AK2A) from cDNA libraries of fetal liver origin. The complete nucleotide sequence of the cloned 889 nucleotide cDNA fragment indicated that the deduced gene product of human AK2A is composed of 239 amino acids with a molecular weight of 26 kD. Comparison of these nucleotide and amino acid sequences with the corresponding sequences of bovine AK2A and rat AK2 revealed a high degree of conservation. It showed 91% and 98% homologies in DNA and amino acid sequences, respectively, with bovine AK2A throughout the full open reading frame. RNA blot analysis revealed that three species of mRNA were present with approximate sizes of 3.4, 2.1, and 1.0 kb. This gene was expressed in E. coli cells and the recombinant protein was enzymatically active.

ISSN 

1039-9712

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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