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Title 

Transcriptional repression of human insulin-like growth factor-II P4 promoter by Wilms' tumor suppressor WT1

Authors 

Young Ik LeeSeong-Jin Kim

Publisher 

Mary Ann Liebert

Issue Date 

1996

Citation 

DNA and Cell Biology, vol. 15, no. 2, pp. 99-104

Keywords 

gene repressionnonhumanpromoter regiontumor suppressor geneearly growth response protein 1humansinsulin-like growth factor IIpromoter regions (Genetics)transcription factorsWT1 prote

Abstract 

The Wilms' tumor suppressor gene wt1 encodes a zinc finger-containing protein that binds to the same DNA sequence as Egr-1, a mitogen-inducible immediate-early gene product that activates transcription. In this study, we investigated whether the human insulin-like growth factor-II (IGF-II) P4 promoter might be a target for transcriptional repression mediated by WT1. Using constructs of the IGF-II P4 promoter linked to the chloramphenicol acetyltransferase gene, we have demonstrated that the WT1 protein represses expression of the IGF-II gene through a GCGGGGGAG response element spanning nucleotides -87 to -65 of the IGF-II P4 promoter. Conversely, we have shown that the Egr-1 activates transcription of the IGF-II gene through the same response element. WT1 and Egr-1 proteins interact directly with the WT1/Egr- 1 response element of the IGF-II promoter 4 in gel mobility-shift assays. These findings demonstrate the importance of the WT1/Egr-1 consensus element for the expression of the IGF-II gene in response to positive or negative transcription signals.

ISSN 

1044-5498

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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