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Title 

Interleukin-4 induced proliferation in normal human keratinocytes is associated with c-myc gene expression and inhibited by genistein

Authors 

Young YangHyun Mi YooIn Pyo ChoiKwang Ho PyunSi Myung ByunHyunjung Ha

Publisher 

Nature Publishing Group

Issue Date 

1996

Citation 

Journal of Investigative Dermatology, vol. 107, no. 3, pp. 367-372

Keywords 

cell cycletyrosine kinasegenisteininterleukin 4okadaic acidrecombinant interleukin 4cell proliferationcontrolled studyhuman cell

Abstract 

We studied the effect of IL-4 on the proliferation of cultured normal human keratinocytes. Keratinocyte proliferation was stimulated by IL-4 and inhibited by anti-IL-4 antibody in a concentration-dependent manner. Anti-IL-6 antibody did not inhibit normal human keratinocyte proliferation, suggesting that the IL-4 could directly induce proliferation of these cells. IL-4 significantly induced cell cycle G0/G1 to S phase progression. The keratinocyte proliferation by IL-4 was mediated through one of the growth control genes, c-myc protooncogene. The expression of c-myc mRNA was significantly increased after IL-4 treatment of the keratinocytes, suggesting that c-myc plays a key role in the control of proliferation. The signal transduction pathways induced by IL-4 in the keratinocytes were studied with inhibitors of signal transduction. Genistein, a tyrosine kinase inhibitor, suppressed the level of the induced c-myc mRNA expression, but H7, a serine/threonine kinase inhibitor, and okadaic acid, a protein phosphatase 1 and 2A inhibitor, did not block the induced c-myc gene expression. Taken together, these results suggest that IL-4 stimulates the proliferation of keratinocytes in vitro by promoting a transition from G0/G1 to S phase of the cell cycle. Induction of c-myc after IL-4 treatment could indicate an important role for c-myc in the proliferation of keratinocytes. Our observations also suggest that tyrosine kinases may be involved in IL-4-induced proliferation.

ISSN 

0022-202X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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