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Title 

Genomic heterogeneity in clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

Authors 

Jeong Ran KimBong Seok KangJung Hun KohJ S ParkS J KimG H BaiT H ChungK S NamYong Kyung ChoeIn Seong ChoeTai Wha ChungYoung Choon LeeChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Journal of Biochemistry and Molecular Biology, vol. 29, no. 6, pp. 569-573

Keywords 

genomemycobacteriapulsed-fielded gel electrophoresis

Abstract 

Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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