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Title 

An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme

Authors 

Ju Won KwakYoung Gyo SeoKi Bang SongSang Ki Rhee

Publisher 

Springer

Issue Date 

1996

Citation 

Biotechnology Techniques, vol. 10, no. 2, pp. 127-132

Abstract 

Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli (Song, K.-B. and Rhee, S.-K. (1994) Biotechnol. Lett. 16, 1305-1310). The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.

ISSN 

0951-208X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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