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Title 

An efficient expression of human growth hormone(hGH) in the milk of transgenic mice using rat β-Casein/hGH fusion genes

Authors 

Chul Sang LeeK J KimDae Yeul YuKyung Kwang Lee

Publisher 

Humana Press

Issue Date 

1996

Citation 

Applied Biochemistry and Biotechnology, vol. 56, no. 3, pp. 211-222

Keywords 

hGHmilkrat β-caseintransgenic micegrowth hormoneanimalgene transferhumantransgenic mousegene transfer techniques

Abstract 

In order to produce human growth hormone (hGH) in the milk of transgenic mice, two expression vectors for hGH differing in their 3' flanking sequences were constructed by placing the genomic sequences of hGH gene under the control of the rat β-casein gene promotor. The 3' flanking sequences of the expression constructs were derived from either the hGH gene (pBCN1GH) or the rat β-casein gene (pBCN2GH). Transgenic lines bearing pBCN1GH expressed hGH more efficiently than those bearing pBCN2GH in the milk (195500 μg/mL vs 0.7-2 μg/mL). In particular, one of the BCN1GH lines expressed hGH as much as 5500 ±620 μg/mL. Northern blot analysis showed that the transgene expression was specifically confined to the mammary gland and developmentally regulated like the endogenous mouse β-casein gene in the mammary gland. However, a low level of nonmammary expression was also detected with more sensitive assay methods. In conclusion, the rat β-casein/hGH fusion gene could direct an efficient production of hGH in a highly tissue-and stagespecific manner in the transgenic mice and the 3' flanking sequences of hGH gene had an important role for the efficient expression.

ISSN 

0273-2289

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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