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Title 

Somatic embryogenesis and plant regeneration in cotyledonary explant cultures of Chinese cabbage

Authors 

Pil S ChoiWoong Y SohJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Plant Cell Tissue and Organ Culture, vol. 44, no. 0, pp. 253-256

Keywords 

brassica campestris ssp. napus pekinensistissue culture

Abstract 

Cotyledonary explants of Chinese cabbage were cultured on Murashige and Skoog's medium supplemented with various concentrations of 2,4- dichlorophenoxyacetic acid. Up to 20% of the cotyledonary explants produced somatic embryos with or without intervening callus production. Explants became more competent as the age of the source seedlings increased up to 8 days, but cotyledonary explants from 10-day-old seedlings were not responsive. Upon transfer to MS basal medium most of the somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity in a phytotron. Among three cultivars used, only cotyledonary explants of 'Top Salad' were capable of producing somatic embryos.

ISSN 

0167-6857

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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