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Title 

M phase-specific phosphorylation of DNA topoisomerase IIα in HeLa cells

Authors 

Young Seuk BaeSool Ja LeeSang Soo Kwak

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Journal of Biochemistry and Molecular Biology, vol. 29, no. 1, pp. 27-31

Keywords 

anti-peptide antibodycell cycleDNA topoisomerase IIprotein phosphorylation

Abstract 

Using topoisomerase II (topo II) isozyme-specific antibodies, we investigated the phosphorylation of topo IIα in mitotic HeLa cells. Topo lia was specifically modified in the mitotic cells, resulting in slow migration on SDS-polyacrylamide gel electrophoresis. To characterize the nature of this modification, we treated the nuclear extracts prepared from the mitotic cells with alkaline phosphatase. After the treatment with alkaline phosphatase, the slowly migrated band disappeared and instead a normal (170 kDa) topo IIα band appeared. These results indicate that human topo IIα is modified at a specific site(s) in M phase by phosphorylation, supporting the possibility that M phase-specific phosphorylation of topo II is critical for mitotic chromosome condensation and segregation.

ISSN 

1225-8687

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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