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Title | New anthracycline metabolites produced by the aklavinone 11-hydroxylase gene in Streptomyces galilaeus ATCC 31133 |
Authors | Hang Sub Kim; Young Soo Hong; Young Ho Kim; O J Yoo; Jung Joon Lee |
Publisher | Nature Publishing Group |
Issue Date | 1996 |
Citation | Journal of Antibiotics, vol. 49, no. 4, pp. 355-360 |
Keywords | 11 hydroxyaclacinomycin x; anthracycline derivative; genetic transformation; streptomyces; snthracyclines; aryl hydrocarbon hydroxylases |
Abstract | Transformation of Streptomyces galilaeus ATCC 31133 with the aklavinone 11-hydroxylase gene (dnrF) resulted in the production of many red pigments. The new metabolites were purified and their structures were determined as 11-hydroxylated aclacinomycin A, B and T by spectral analysis. This result indicated that the dnrF was stably expressed in the strain S. galilaeus ATCC 31133 to give rise to hybrid aclacinomycins. In addition, a new aclacinomycin analog named 11-hydroxyaclacinomycin X was isolated from the same culture. Its structure was elucidated as 2'''-amino-11-hydroxyaclacinomycin Y. It showed strong cytotoxicity against several human tumor cell lines, especially leukemia and melanoma cell lines. |
ISSN | 0021-8820 |
Appears in Collections | |
Registered Date |
2017-04-19 |
