상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Isolation and characterization of MT2617-2B, a phospholipase C inhibitor produced by an actinomycetes isolate

 

방선균 분리주가 생산하는 Phospholipase C 저해물질인 MT2617-2B의 분리 및 특성

Authors 

Hack Ryong KoHyun Sun LeeWon Keun OhSoon Cheol AhnBo Yeon KimDae Ook KangTae Ick MheenJong Seog Ahn

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1996

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 24, no. 1, pp. 19-26

Keywords 

inhibitor of phospholipase CmacrolideMT2617-2Bsignal transduction

Abstract 

A phospholipase C (PLC) inhibitor (MT2617-2B) was isolated from the culture broth of actinomycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR, 13C- and 1H-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of IC50, against PLC-γ1 and PLC-β1 were 25 and 50 μg/ml, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)