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Title 

Molecular cloning and induction of β-1,3-glucanase gene from Nicotiana glutinosa L.

Authors 

Hae Keun YunSo Young YiSung Uk KimKwang Hee SonSeung Hun YuSong Hae BokDo Il Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Molecules and Cells, vol. 6, no. 4, pp. 422-428

Abstract 

Cloning and characterization of disease-response genes in plants could be an initial step toward understanding the complex disease resistance mechanism. To better understand the complex step, we isolated one of the pathogenesis-related proteins, β-1,3-glucanase, cDNA from a cDNA library of Nicotiana glutinosa showing systemic resistance. One clone (GN-3) was a partial cDNA of β-1,3-glucanase 800 bp in size with a 171 amino acid coding region. This clone had a 90% nucleotide homology with the β-1,3-glucanase gene of N. tabacum cv. BY4. A deduced amino acid sequence of GN-3 clones indicated a 91% identity with the β-1,3-glucanase of tobacco, 58% with that of Lycopersicon esculentum, and 51% with that of Glycine max. Northern blot analysis showed that expression of β-1,3-glucanase mRNAs was induced by TMV infection and salicylic acid treatment. In addition to that this gene was highly induced by CuSO4 and β-aminobutyric acid which are known as inducers of plant disease resistance. The possible role of this gene expression in relation to chemical-induced plant defense responses is discussed.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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