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Title 

Purification and characterization of a thermostable alkaline protease from Thermoactinomyces sp. E79 and the DNA sequence of the encoding gene

Authors 

Jung Kee LeeYoung Ok KimHyung Kwoun KimY S ParkTae Kwang Oh

Publisher 

Japan Society for Bioscience, Biotechnology and Agrochemistry

Issue Date 

1996

Citation 

Bioscience Biotechnology and Biochemistry, vol. 60, no. 5, pp. 840-846

Keywords 

amino acid sequence similarityDNA sequenceprotein purificationthermoactinomyces sp.thermostable alkaline proteasebacterial DNAmicrobial serine proteinasesserine proteinaseamino acid sequenceclassification

Abstract 

A thermophilic Thermoactinomyces sp. E79 producing a highly thermostable alkaline protease was isolated from soil. The protease, produced extracellularly by Thermoactinomyces sp. E79, was purified by DEAE- Sepharose CL-6B and Butyl-Toyopearl 650M column chromatography. The relative molecular mass was estimated to be 31,000 by SDS-polyacrylamide gel electrophoresis. Enzyme activity was inhibited by phenylmethylsulfonyl fluoride, suggesting the enzyme to be a serine protease. The optimum temperature for the enzyme activity was 85°C, and about 50% of the original activity remained after incubation at 90°C for 10min in the presence of Ca2+. The optimum pH for the enzyme activity was 11.0 and the enzyme was fairly stable from pH 5.0 to 12.0. The gene for this thermostable alkaline protease was cloned in Escherichia coli and the expressed intracellular enzyme was activated by heat treatment. Sequence analysis showed an open reading frame of 1,152 base pairs, coding for a polypeptide of 384 amino acids. The polypeptide was composed of a signal sequence (25 amino acids), a prosequence (81 amino acids), and a mature protein of 278 amino acids. The deduced amino acid sequence of the mature protease had high similarity with thermitase, a serine protease from Thermoactinomyces vulgaris, and the extent of sequence identity was 76%.

ISSN 

0916-8451

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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