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Title 

Characterization of an alkaline lipase from Proteus vulgaris K80 and the DNA sequence of the encoding gene

Authors 

Hyung Kwoun KimJung Kee LeeHyoung Man KimTae Kwang Oh

Publisher 

Wiley-Blackwell

Issue Date 

1996

Citation 

FEMS Microbiology Letters, vol. 135, no. 0, pp. 117-121

Keywords 

alkaline enzymeenterobacteriumlipase genelipase K80proteus vulgarisalkaline lipasetriacylglycerol lipaseamino acid sequencedna sequence

Abstract 

A facultatively anaerobic bacterium producing an extracellular alkaline lipase was isolated from the soil collected near a sewage disposal plant in Korea and identified to be a strain of Proteus vulgaris. The molecular mass of the purified lipase K80 was estimated to be 31 kDa by SDS-PAGE. It was found to be an alkaline enzyme having maximum hydrolytic activity at pH 10, while fairly stable in a wide pH range from 5 to 11. The gene for lipase K80 was cloned in Escherichia coli. Sequence analysis showed an open reading frame of 861 bp coding for a polypeptide of 287 amino acid residues. The deduced amino acid sequence of the lipase gene had 46.3% identity to the lipase from Pseudomonas fragi.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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