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Title 

Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli

Authors 

Hyung Kwoun KimYoung Seo ParkHyoung Man KimTae Kwang Oh

Publisher 

Japan Society for Bioscience, Biotechnology and Agrochemistry

Issue Date 

1996

Citation 

Bioscience Biotechnology and Biochemistry, vol. 60, no. 8, pp. 1365-1367

Keywords 

interfacial activationlidlipaseproteus vulgaristriacylglycerol lipaseescherichia coli

Abstract 

An expression plasmid carrying an alkaline lipase gene from Proteus vulgaris was constructed. The lipase content in Escherichia call cells harboring the expression plasmid reached about 22% of total soluble protein. The purified enzyme displayed a partial interfacial activation toward p- nitrophenyl butyrate (PNPB).

ISSN 

0916-8451

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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