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Title 

Optimal conditions for levan formation by an overexpressed recombinant levansucrase

Authors 

H BelghithKi Bang SongChul Ho KimSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Biotechnology Letters, vol. 18, no. 4, pp. 467-472

Keywords 

levanlevansucrase

Abstract 

A genetically modified levansucrase, which contained His-affinity tag in its C-terminal, was constructed by PCR reaction using two synthetic primers. This modified protein was produced up to 30% in total cell protein of E. coli, and was purified by a one-step affinity chromatography. The optimum pH for levan production was pH 5 and the optimum temperature was 0°C. The higher velocity of levan formation within shorter enzyme reaction times was achieved by increasing the levels of enzyme concentration. The optimal sucrose concentration for levan production was around 20%. Under these conditions, more than 50 g levan/l was produced.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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