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Title 

Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase

Authors 

Seung Goo LeeSeung Pyo HongMoon Hee Sung

Publisher 

Elsevier

Issue Date 

1996

Citation 

Enzyme and Microbial Technology, vol. 19, no. 0, pp. 374-377

Keywords 

enzymatic removal of phenolthermostable β-tyrosinasewastewater treatmentamino acidsammoniabiocatalystsbioconversionchemicals removal (water treatment)manufacturephenolic resins

Abstract 

This study explores an enzymatic method for removing phenol from the wastewater created during the manufacture of phenolic resin. The enzyme used was a thermostable β-tyrosinase catalyzing the synthesis of L-tyrosine from phenol, pyruvate, and ammonia. As the reaction proceeds, L-tyrosine precipitates as insoluble aggregates because L-tyrosine is barely soluble in water. The enzymatic removal of phenol was effective at pH values ranging from 6.5-9.0 and temperatures below 70°C. The optimal concentration of each substrate was determined as 60 mM phenol, 0.1 M pyruvate, and 0.4 M ammonia. When the enzyme was used in an intact cell or acetone-dried cell state instead of the cell-free extract, the optimal concentration of phenol was increased up to 120 mM. By treating wastewater containing 100 mM phenol with acetone-dried cells at 37°C, we could reduce the concentration of phenol to 8 mM within 24h.

ISSN 

0141-0229

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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