상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Cloning and expression of a Bacillus sp. 79-23 cellulase gene

Authors 

Kyung Hwa JungYoung Chin ChunJae-Chan LeeJeong-Hyun KimKi-Hong Yoon

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Biotechnology Letters, vol. 18, no. 9, pp. 1077-1082

Keywords 

carboxymethylcellulosecellulasemicrocrystalline cellulosepyranosidebacillusenzyme activityescherichia colimolecular cloning

Abstract 

A gene for encoding cellulase was cloned from Bacillus sp. 79-23 into Escherichia coli and the nucleotide sequence was determined. The cellulase gene, designated as celS, was composed of 1,497 base pairs and the nucleotide sequence of the celS gene was highly homologous to those of other B. subtilis cellulase genes. The enzyme encoded by celS was highly active on carboxymethylcellulose but also exhibited activity towards avicel and p-nitrophenyl-β-D-cellobiopyranoside. When its native promoter was replaced with a strong B. subtilis promoter, the extracellular cellulase was produced up to 8.5 units per ml in B. subtilis DB104.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)