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Title 

Effects of reaction additives on enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine

 

3,4-dihydroxyphenyl-L-alanine의 효소적 생산에 대한 반응첨가물의 영향

Authors 

Seung Goo LeeHyeon Su RoSeung Pyo HongMoon Hee Sung

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

1996

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 24, no. 2, pp. 222-226

Keywords 

L-DOPAorganic solventpyridoxal-5-phosphatesodium boratetyrosine phenol-lyase

Abstract 

The enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) was examined for the effects of the reaction additives such as sodium borate, alcohol, and organic solvents. The enzyme used was tyrosine phenol-lyase of Citrobacter fretindii KCTC 2006 produced in Escherichia coli. The amounts of tyrosine phenol-lyase and pyridoxal-5-phosphate were optimized to 2.0 units/ml and 0.1 mM, respectively, for the synthetic reaction. Sodium borate, a substance that forms a complex with pyrocatechol, reduced the enzyme deactivation by pyrocatechol although it seriously inhibited the enzyme activity. Among the organic solvents tested, dimethylsulfoxide, dimethylformamide, and alcohol increased the productivity of the L-DOPA synthesis. In a reaction system with 5% methanol, L-DOPA concentration increased up to 210 mM after 24 hours, and 77.1% of which was separated as precipitates. The L-DOPA was purified to 99.96% by solubilizing and recrystallyzing the precipitates.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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