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Title 

Enzymatic conversion of glutaryl 7-aminocephalosporanic acid to 7-aminocephalosporanic acid with an immobilized glutaryl 7-aminocephalosporanic acid acylase

Authors 

Han Jae ShinSeung Goo LeeWang-Sik LeeKi Hong Yoon

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1996

Citation 

Journal of Microbiology and Biotechnology, vol. 6, no. 5, pp. 336-339

Keywords 

7-aminocephalosporanic acidglutaryl 7-aminocephalosporanic acid acylaseimmobilizationpseudomonas sp.

Abstract 

Ghitaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30% of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be 30°C and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 ml of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60 mM GL 7-ACA within 8 h, resulting in a conversion yield of 83%.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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