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Title 

Cryopreservation of Scutellaria baicalensis cells by two-step cooling method

Authors 

W T SwoSuk Weon KimJang Ryol LiuIk Hwan KimYoung Hoon ParkT B Choe

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1996

Citation 

Journal of Microbiology and Biotechnology, vol. 6, no. 3, pp. 209-212

Keywords 

BaicalinCell suspension cultureCryopreservationScutellaria baicalensis GeorgiTwo-step cooling

Abstract 

A two-step cooling technique has been developed for cryopreservation of suspension cultured Scutellaria baicalensis cells. Efficient regrowth of cryopreserved cells was obtained in cryoprotected cells with a mixture of 1.5 M glycerol and 0.4 M sucrose in Schenk and Hildebrandt medium without pretreatment in high osmotic medium. Optimum freezing conditions were found to be a cooling rate of 0.5°C/ min from 4°C to -40°C, and then retaining samples at -40°C for 30 min prior to plunging into liquid nitrogen. A regrowth rate of approximately 95% was obtained after three month storage in liquid nitrogen. Callus cultures established from the cryopreserved cells were found to produce the same patterns of flavonoid accumulation and retain their baicalin producing activity.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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