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Title 

High cell density cultivation of Pseudomonas putida BM01 using glucose

Authors 

Guk Jin KimIn Young LeeDae Keon ChoiSung Chul YoonYoung Hoon Park

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1996

Citation 

Journal of Microbiology and Biotechnology, vol. 6, no. 3, pp. 221-224

Keywords 

GlucoseHCDCHigh density cell cultivationPesudomonas putida

Abstract 

Pseudomonas putida BM01 was grown efficiently on glucose as the sole carbon source with a supply of a nitrogen source in pH-stat mode using a low setpoint limit. A final cell concentration of 100 g/l was obtained in 30 h of fed-batch cultivation by controlling glucose concentration within the range of 5-20 g/l and maintaining dissolved oxygen tension above 10% saturation using pure oxygen. This high cell density culture technique is believed highly useful for the production of poly(3-hydroxyalkanoates) by this strain.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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