상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Down-regulation of protein kinase C in murine splenocytes : a potential mechanism for 2-acetylaminofluorene-mediated immunosuppression

Authors 

Michael LeeHwan Mook KimKyu-Hwan Yang

Publisher 

Elsevier

Issue Date 

1996

Citation 

Cancer Letters, vol. 101, no. 0, pp. 53-57

Keywords 

2-acetylaminofluoreneImmunosuppressionProtein kinase C

Abstract 

2-Acetylaminofluorene (AAF), an arylamide carcinogen, inhibited in a dose dependent manner mouse spleen cell proliferation in response to lipopolysaccharide (LPS). The objective of the present studies was to investigate the effects of AAF on protein kinase C (PKC) activation, an enzyme required for LPS-induced splenocytes proliferation. After treatment with 50μM AAF for 18 h, PKC activity in the cytosolic fraction decreased by 50% from the control level, and splenocytes lost 30% of total PKC activity. Furthermore, as determined by the electrophoretic mobility shift assay, AAF inhibited the binding activity of the transcription factor complex, NF-κB, whose LPS-mediated induction is dependent on PKC activation in murine splenocytes. These results strongly suggest that LPS-mediated signaling in spleen cells is interrupted by AAF early in the signal transduction pathway, at a point proximal to the activation of PKC.

ISSN 

0304-3835

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)