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Title 

A humanized antibody with specificity for hepatitis B surface antigen

Authors 

Chun Jeih RyuE A PadlanByung Rae JinOok Joon YooHyo Jeong Hong

Issue Date 

1996

Citation 

Human Antibody Hybridomas, vol. 7, no. 3, pp. 113-122

Keywords 

Antibody engineeringHBV neutralizationHumanized antibodyProphylaxisamino acidhepatitis b surface antigenmonoclonal antibodyamino acid sequenceantibody specificityantibody structure

Abstract 

A murine monoclonal antibody H67 was characterized for the binding specificity which showed that H67 recognizes a disulfide-bond-dependent conformational epitope of common a antigenic determinant on the hepatitis B surface antigen. The result suggested that this antibody may have the potential of replacing hepatitis B immune globulin in the prevention of hepatitis B virus (HBV) infection. Therefore, we have constructed the humanized antibody HuS10 by grafting the complementarity determining regions and some framework amino acid residues of H67 onto the most homologous human antibody variable regions, 21/28 for heavy chain variable region and B1 and J(k)2 for light chain variable region, followed by combining with human constant regions C(y)1 and C(k). The affinity of the HuS10 was the same as that of the H67, 8 x 108 M-1, and the HuS10 neutralized the in vitro infection of adult human hepatocyte primary culture by adr or ayw subtype of HBV. We neutralization assay showed that the HuS10 had approximately 2,000-times higher specific activity than commercially available polyclonal HBIG. These results suggest that the humanized antibody will be useful in the prevention or treatment of HBV infection.

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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