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Title 

Cloning and characterization of chitin synthase gene fragments from Penicillium chrysogenum

Authors 

Namgung JunBum Chan ParkDong Hoon LeeKyung Sook BaeHee-Moon Park

Publisher 

Wiley-Blackwell

Issue Date 

1996

Citation 

FEMS Microbiology Letters, vol. 145, no. 0, pp. 71-76

Keywords 

Chitin synthase genesPenicillium chrysogenumPolymerase chain reactionchitin synthasegenemolecular cloningpenicillium chrysogenumChitin SynthaseCloning, MolecularGenes, Bacterial

Abstract 

DNA fragments homologous to chitin synthase were amplified from the genomic DNA of Penicillium chrysogenum by PCR. Cloning and sequencing of the PCR-amplified fragments led to the identification of four different genes, designated PcCHS1, PcCHS2, PcCHS3, and PcCHS4. By comparison of the deduced amino acid sequences, PcCHS1 was identified as a gene for class I chitin synthase, PcCHS2 and PcCHS3 were for class II, and PcCHS4 was for class III. Among these only PcCHS4 includes an intervening sequence of 56 bp. The analysis of the deduced amino acid sequences revealed a close evolutionary relationship between Penicillium and ascomycetous fungi.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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